A Study On Nutrient Agar - 1078 Words

Nutrient Agar To observe the colony morphology, the original unknown culture was streaked for isolation on nutrient agar using the quadrant streaking technique, inverted, and incubated at 37 ° C for 48 hours. This allowed for observation of the colony morphology. Separation of Gram-positive and Gram-negative The original unknown culture was streaked for isolation on Columbia CNA agar and MacConkey agar using a quadrant streak, inverted, and placed in a 37 ° C incubator for 48 hours. CNA agar contains a mix of colistin and nalidixic acid, as well as sheep blood. If there is poor or no growth on CNA agar, then the organism was inhibited by colistin and nalidixic acid and is Gram-negative. However if there is good growth, the organism was not inhibited by colistin and nalidixic acid, and is Gram-positive. MacConkey agar contains bile salts and crystal violet. If there is poor growth or no growth, the organism was inhibited by crystal violet and/or bile, and is Gram-positive. If there is good growth, the organism was not inhibited by crystal violet or bile, and is Gram-negative. Gram Stain This protocol was performed once using the culture on the Columbia CNA agar, and once using the culture on the MacConkey agar. The Gram stain uses crystal violet as a primary stain, ethanol-acetone as a negative stain, and safranin as a counterstain. A small amount of the culture was heat-fixed to a glass microscope slide. The culture was flooded with methylene blue, and allowed to sit for oneShow MoreRelatedA Research Study On Nutrient Agar1678 Words   |  7 Pagesdiarrhoea, myalgia, hypotension and signs of septic shock. In order to identify possible bacteria responsible for these symptoms, a vaginal swab was sampled, incubated on agar overnight and stored at 4C, after which they were examined throughout a series of microbiological tests. The initial culturing of the sample on nutrient agar indicated two organisms were present, and hence these were analysed individually. A Gram stain was applied to each bacterium and they were viewed individually under microscopeRead MoreWhy Are Microbes So Important?944 Words   |  4 Pagesbacteria, algae, fungi and protozoan were recorded. Materials and Methods Nutrient Broth and Nutrient Agar were used to inoculate bacteria taken from different surfaces. Nutrient agar plate was inoculated with a sample taken from skin surface. A sterile cotton swab was first immersed on sterile water, then, rubbed against the skin with swirling motion and transferred to an agar plate by rubbing it against the surface of the agar. The plate was incubated at 37 ËšC for 48 hours. Followed Experiment 1fromRead MoreThe Theory Of Microorganisms Exist1470 Words   |  6 PagesIntroduction Microbiology is the study of microorganisms; whether it is bacteria, fungi, algae, protozoan, viruses or archaea (Leboffe Pierce, 2006). Microbes come in all different structures and classifications. Before the 17th century the idea or microbes existed, but was never proven until a scientist provided something that allowed us to see what the naked eye alone could not see (Pelczar, 2015). It all began when Anton van Leeuwenhoek invented a microscope and began observing objects underRead MoreEssay On Gene Expression In Bacteria1310 Words   |  6 Pagescertain genes to be â€Å"turned on† or â€Å"turned off†. Although genes are always present in the genome, various stimulants or inhibitors may cause gene expression (i.e. which genes are turned on or off). To begin this experiment, I acquired sterile nutrient agar from Mr. Goble (shoutout). I also acquired two sterile petri dishes that were included in the kit I received. On the bottom of one, I wrote in red expo marker â€Å"A 25o C Nat† and on the other I wrote â€Å"A 37o C Nat†. I then placed Mr. Goble’s largeRead More Common Microorganisms Essay1000 Words   |  4 Pagesorganisms can easily be seen using differing types of agar, which creates an ideal environment for the organisms to form colonies, which are groups of hundreds of organisms that can be seen with the naked eye. In order to see individual microorganisms, it is necessary to use the magnification of a high-powered microscope. These techniques of microbiology are used in the following five experiments. The first experiment used Trypticase Soy Nutrient Agar (TSA), which can grow a wide variety of organismsRead MoreEffect of Sugar on Bean Plant Growth1624 Words   |  7 Pagesof water would help bean plants grow to be stronger, healthier and larger because they would get energy from the sugar. Methods/Materials 36 bean plants were grown in potting soil. The same amount of soil was used in each pot and it had no added nutrients. The plants were grown under 24 hour light, for the same length of time. They were watered everyday with 22.18 milliliters of water. The amount of sugar in the water was either: no sugar, (control), 25 grams of sugar per liter of water, 50 gramsRead MoreThe Organisms And Its Effects On Our Daily Environmental Surfaces And Primary Areas Of Life Essay1711 Words   |  7 Pagesgrowth be greater than samples taken from the human body? Material and Methods Students were provided with the following equipment in a sterilized Microbiology Lab. Cotton Swabs †¢ Distilled Water †¢ Test Tubes with caps †¢ Petri Dishes †¢ Nutrient Agar †¢ Blood Agar †¢ Inoculation Loop †¢ Bunsen Burner †¢ Slides †¢ Pipette †¢ Compound Light Microscope †¢ Various Reagents. Students were asked to gather samples from four sites on a college campus where it was believed bacteria would be most concentrated. StudentsRead MoreThe Medical Microbiology Is An Aspect Of Microbiology Sciences1080 Words   |  5 PagesBacteria can also cause disease from an infection of the opportunistic bacteria living symbiotically with people or foreign bacteria introduced from various plausible sources. The identification of the unknown bacteria was accomplished by an independent study of numerous biochemical tests. The tests gave physiological information about the morphology, type of respiration, energy sources, and metabolic processes. The test results indicated the bacteria was Escherichia coli. Materials Methods Unknown microorganismRead MoreChemical Chemicals : A Study And Their Manufacturing Companies Essay995 Words   |  4 PagesAll chemicals used in this study are of analytical grade, Table 3.1 shows the chemicals used in the study and their manufacturing companies. Table 3.1 Chemicals used in the present study. Chemicals Manufacturing companies Sodium acetate Sigma, USA Yeast extract Lab M, UK Agar B V srl-Parma, Italy Acetic acid ADWIC, Egypt Glucose Sigma, USA GABA Loba, India MSG Sigma, USA DEAE-Cellulose Sigma, USA Catalase enzyme Biodiagnostic, Egypt APTT kit PT kit Biomed diagnostics, Germany n-butanol SigmaRead MoreAshitaba as an Alternative to Commercial Hand Sanitizer587 Words   |  3 Pagesproperly followed to gather the needed information and necessary data for the study. A. Sources of Test Organisms The bacterial isolates (Staphylococcus aureus, Escherichia coli and Streptococcus) used in the experiment were obtained from Institute of Biological Science, University of the Philippines Los Baà ±os, Laguna. B. Maintenance of Microbial Cultures Bacterial isolated were subculture on slants of Nutrient Agar (NA). Incubation of the subcultured specimens for bacteria was done for